Survey on methods used for the detection of carbapenemase-producing organisms in clinical diagnostic medical microbiology laboratories

UK NEQAS are investing into developing a new EQA scheme for detection of carbapenemase-producing organisms.

In 2013 Public Health England published, Acute trust toolkit for early detection, management and control of carbapenemase-producing Enterobacteriaceae.

In 2013 EUCAST subcommittee published a recommendation for detection of resistance mechanisms of CPE with clinical and/or epidemiological importance.

In September 2016 SMI for detection of bacteria with carbapenem-hydrolysing β- lactamases have been published.

There is an urgent need for accurate and fast detection of carbapenemases in diagnostic laboratories.

In this survey, we request information on the screening methods for suspected isolates, direct assays for confirmation of carbapenemase activity (e.g. the Carba NP test and matrix-assisted laser desorption ionization time-of-flight mass spectrometry carbapenem hydrolysis assay), inhibitor-based methods for carbapenemase classification, and molecular-genetic techniques for precise identification of carbapenemase genes.

Please complete and return this survey to determin the algorithm in the identification of carbapenemase-producing organisms used by diagnostic laboratories to aid development of a new UK NEQAS EQA scheme.

This potential new scheme will provide the much needed assurance of your internal protocol and improve patient care.

Thank you in advance for your contributions in providing the information requested.

Please return results as soon as possible, but not later than 18/08/2017.

Laboratory Identification Number:*
E-mail:*
Question 1
Do you have an established screening protocol for detection of carbapenemase-producing organism in your laboratory?
Question 2
How do you assure carbapenemase-producing organisms are not missed
Please specify:
Question 3
What group of patients do you test?
Question 4
What type of clinical specimens do you test for carbapenemase?
Question 5
Screening methodology:
Question 6
Phenotypic tests:
Question 7
Detection based on hydrolysis:
Question 8
Do you use molecular-genetic techniques for carbapenemase detection directly from specimens:?
Please specify:
Question 9
How do you confirm potential carbapenemase detection?
Name of Reference Laboratory
Please specify the method:
Please specify:
Comments:
Word Verification:

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