Questionnaire Feedback Questionnaire on Water & Environmental sample testing for Legionella spp. External Quality Assessment (EQA) scheme for supporting the surveillance of Legionnaires’ disease at European levelAdditional questions associated with the water and environmental sample testing for this EQA exercise for Legionella spp.Instructions:The below information should be provided on-line when you return your results for this EQA exercise distribution. Lab ID No:* Email:* Full Name:*FirstLast Organisation:*General Information: Approximately how many samples in a year does your laboratory examine for Legionella spp.? Select value<1010-100100-500500-1000>1000 What types of sample are these? (Click all that apply)Cooling towersSpa waterHot and cold water systemsBathing watersSwabs from biofilmsOther Does your laboratory participate in a National EQA scheme for the detection of Legionella species? *YesNo Is this a voluntary participation or a mandatory requirement?*VoluntaryMandatoryNot applicableEQA Distribution Questions:Methods for Water Isolation: What published method do you use for examining water samples for Legionella spp.?ISO 11731:2017ISO 11731-2:2004ISO 11731-2:2008LegiolertAFNOR NF T90-431:2017Other What published method do you use for examining swab samples for Legionella spp.? ISO 11731:2017Other What method/s was employed to examine the samples in this exercise? (Click all that apply)FiltrationCentrifugationNeatOther What volume was used to examine the water samples? 100mL500mL1000mLOther What volume of eluent was used to resuspend the bacteria from the membrane? What was the volume of the working concentrate? Did you perform neat/ heat/ acid/ testing of the sample? (Click all that apply)NeatHeatAcidOther At what stage of the processing was acid applied? (Click all that apply)After centrifugationOn concentrated sampleAfter filtration and bacterial released from membraneDirectly on membraneOther What culture media was used for isolation of legionella from the sample? (Click all that apply)BCYEBCYE with antibiotics GVPCMYWOther Was the media used commercial bought or made in house? CommercialIn-houseOther What was the lowest detection limit for the method you used? Confirmation tests done: What confirmation tests were done on the isolates? (Click all that apply)Subculture on BCYESubculture on BCYE with cysteinePCROther What culture media were used for confirmation? BCYEKBCY-CCBA Other If your water method differs for swabs, please describe the differences? Information on any molecular methodsused: Briefly describe your concentration process used for the samples. Provide the details of your DNA extraction method or commercial kit used. Bio-Rad Aquadien™ Bacterial DNA Extraction and PurificationBio-Rad InstaGene™ MatrixRoche Diagnostics MagNA Pure LC DNA Isolation Kit IIIOther What volume of extracted DNA was used for your assay? 5 uL10 uL100 uLOther Provide details on the commercial kit of the assay used. Primerdesign genesig® Kit for Legionella spp. kitBio-Rad iQ-Check® Legionella Real-Time PCR KitBio-Rad iQ-Check® Quanti Legionella spp. and L. pneumophila Real-Time PCR Quantification KitOther Please provide details of quantification standards used. Please describe any controls used. Provide details on the amplification platform used. Applied Biosystems® 7300 Fast Real-Time PCR SystemBio-Rad CFX96 Touch™ Deep Well RT-PCR Detection SystemBio-Rad iQ-Check® Quanti Legionella spp. and L. pneumophila Real-Time PCR Quantification KitOther What were your limits of detection (LOD) (Please describe measure and volume)? What were your limits of quantification (LOQ) (Please describe measure and volume)? Information on serogroup methods: Which kit/method was used to serogroup the isolate? Information on sequence base typing (SBT): Which method was used for SBT? Thank you for your help.Please return completed forms no later than 17:00 16/12/2019. If you have any queries please contact us at organiser@ukneqasmicro.org.uk with the subject header as ECDC Legionella Environmental Questionnaire. Word Verification LID*SubmitReset
