UK NEQAS for Microbiology (SARS COV-2 EQA) Questionnaire for new participants registering on molecular detection of SARS-CoV-2 EQA The purpose of the current survey is to collect information on the general laboratory practices from laboratories planning to participate in the UK NEQAS for Microbiology Molecular detection of SARS-CoV-2 External Quality Assessment All collected information is strictly confidential and used only for analysis purpose. A survey report will be sent to the participating laboratories after all data analysis processes are completed. Contact name:* Job Title:* Name of the laboratory:* Address 1:* Address 2:* City:* Postcode / Zip Code* Country:* Laboratory Code* Contact Email:* Phone Number Including Country Code* Date* 1. Which of the following control measures for preventing cross-contamination is/are implemented in your laboratory?*Separate working rooms/areas for various stages of testingSeparate sets of equipment and consumables for each working room/area Unidirectional workflow from clean to contaminated workUse of hood/station for PCR reagent preparationUse of aerosol-resistant pipette tips and surface decontamination reagents Periodic environmental check (e.g. bench swab check) 2. Which of the following specimen management measures is/are included in the documented procedures for your molecular laboratory?*Specimen collection requirementsSpecimen identification requirementsSpecimen transportation requirementsSpecimen reception proceduresSpecimen storageStandardized test requisition form 3. Which of the following specimen registration systems is/are currently used in your laboratory? *Paper-basedElectronic database system 4. Which of the following examination procedures is/are documented for the molecular detection of SARS-CoV-2?*Procedures for nucleic acid extractionWorkflow for detection of SARS-CoV-2Workflow for genotyping and sequencing of SARS-CoV-2Workflow for handling equivocal results 5. Which type(s) of quality control(s) is/are included in your molecular detection laboratory?*Extraction controlPositive control for PCRNegative control for PCRInternal (inhibitor) control for PCR 6. What method do you use for the extraction of your SARS-CoV-2 specimens?*ManualAutomated Please specify:* Name of Kit used* Reference number of kit* Volume of material required for extraction (µL):* Elution volume (µL):* Expiry date: * 7. Which method of amplification do you use for your molecular method?*Conventional PCRReal time PCRLAMPTMAOther - Please specify:8. Amplification Platform Name:* Model:* 9(a). Amplification kit for detection*In houseCommercialCDCOther 9(b). Please specify amplification kit details: Please specify gene amplified and product size if known: Please specify reaction volume (µL): Please specify volume of nucleic acid template used (µL): 10(a). Genotyping kit*In houseCommercialCDCOther 10(b). Please provide Genotyping kit details: Please specify reaction volume (µL) Please specify volume of nucleic acid template used (µL) 11. Please specify the number of gene targets in your assay:*OneTwoThreeOther - Please specify:12. Please specify gene targets in your assay (e.g. S gene): Target 1: Target 2: Target 3: Other - Please specify: 13. Which of the following examination procedures is/are adopted for the confirmation PCR positive results? *Repeat the detection assay Test with different set of primers/probes Test with different set of primers/probes Forward specimen to a reference laboratory 14. Which of the following post-examination procedures is/are adopted? *Result interpretation for reportingResult counter-checking by another personResult authorization by designated personDesignated reporting formatRetention of raw dataRetention of a copy of the report (electronic or hard copy)Specimen retention policyReporting of urgent results 15. Which of the following safety guidelines is/are implemented in your laboratory?*Appropriate use of personal protective equipmentAppropriate use of personal protective equipmentAppropriate use of safety signage/labelsHandling of chemicalsHandling of chemical spillageHandling of biological/infectious materialHandling of biological spillageDecontamination/disinfection/sterilization proceduresWaste managementPeriodic biosafety drill/exerciseShipping of infectious substances 16. Which of the following safety management measures is/are implemented in your laboratory?*Risk assessmentSafety audit/inspection Policy for incident reporting/follow-upSafety training programme for all staffDesignated officer for safety managementThank you for completing this questionnaire.If you have any queries please contact us at organiser@ukneqasmicro.org.uk with the subject header as SARS-CoV-2 Questionnaire. Word VerificationSubmitReset
